1. DNA ISOLATION OF TRANSGENIC PLANT

2. PURIFICATION OF DNA

3. PCR AMPLIFICATION

4. AGAROSE GEL ELECTROPHORESIS

1. AGAROSE GEL ELECTROPHORESIS

2. SDS-POLY ACRALAMIDE GEL ELECTROPHORESIS

3. NATIVE-POLY ACRALAMIDE GEL ELECTROPHORESIS

4. IMMUNOELECTROPHORESIS

TECHNIQUES INVOLVED IN RAPD ANALYSIS

1. PLANT DNA ISOLATION

2. PURIFICATION OF ISOLATED DNA

3. PCR AMPLIFICATION

4. AGAROSE GEL ELECTROPHORESIS

MODULE I:

1. INTRODUCTION TO GENERAL MICROBIOLOGY

2. BASIC CONCEPTS
   1. Sterilization techniques
   2. Microbiological culture media and its importance
   3. Isolation of microorganisms from soil air and water
   4. Pure culture preparation
   5. Staining techniques

3. BIOCHEMICAL TESTS

4. GROWTH CURVE

5. MUTATIONAL STUDIES BY REPLICA PLATING

6. BACTERIAL CONJUGATION

7. SCREENING FOR DESIRED ORGANISMS

8. ISOLATION AND IDENTIFICATION OF FUNGI

9. PRODUCTION OF SECONDARY METABOLITES

10. ANTIBIOTIC ASSAY

MODULE II

1. INTRODUCTION TO INDUSTRIAL MICROBIOLOGY

2. BASIC CONCEPTS
   1. Sterilization of fermentation equipment.
   2. fermentation systems steps in fermentation
   3. designing of production media

3. SCREENING OF DESIRED ORGANISMS.

4. TYPES OF FERMENTATIONS
   1. solid state fermentation
   2. submerged fermentation

5. DOWN STREAM PROCESSING
   1. Cell separation techniques
   2. Extraction of desired products
   3. Purification of products

6. ANALYSIS OF METABOLITES OR PRODUCTS
1. Chromatography
2. Spectrophotometric determination

MODULE III

1. INTRODUCTION TO ENZYMOLOGY

2. ENZYME PRODUCTION
1. solid state fermentation
2. submerged fermentation

3. ENZYME PURIFICATION
1. salt precipitation
2. Chromatography

4. QUANTITATIVE ESTIMATION OF ENZYME PROTEINS

5. ENZYME KINETICS
1. pH
2. Temperature
3. Substrate concentration

6. SDS-PAGE

7. Zymography (theory)

MODULE IV

1. INTRODUCTION TO IMMUNOLOGY

2. GEL DIFFUSION TECHNIQUES
   1. Ouchterlony Double Diffusion (ODD)
   2. Single Radial Immuno Diffusion (RID)

3. IMMUNO ELECTROPHORESIS
   1. Counter Current Electrophoresis (CCE)
   2. Rocket Immuno Electrophoresis (RIE)

4. ISOLATION OF IgG FROM BLOOD

5. PURIFICATION OF IgG
   1. Salt precipitation
   2. Chromatography
   3. Quantitative estimation of proteins

6. SEPARATION OF LYMPHOCYTES

7. AGGLUTINATION REACTIONS              
   1. Blood grouping and Rh typing
   2. Rapid plasma reagin test (VDRL)
   3. Pregnancy test

8. ELISA (STRIP TEST)

MODULE V

INTRODUCTION TO r-DNA TECHNOLOGY

1. ISOLATION OF GENOMIC DNA
   1. Plant
   2. animal

2. ISOLATION OF TOTAL RNA

3. QUALITATIVE AND QUANTITATIVE ANALYSIS OF NUCLEIC ACIDS

4. PCR TECHNIQUES

5. ISOLATION OF PLASMID DNA

6. AGAROSE GEL ELECTROPHORESIS

7. RESTRICTION DIGESTION

8. TRANSFORMATION

9. LIGATION

MODULE I:

1. INTRODUCTION TO GENERAL MICROBIOLOGY

2. BASIC CONCEPTS   
   1. Sterilization techniques
   2. Microbiological culture media and its importance
   3. Isolation of microorganisms from soil air and water
   4. Pure culture preparation
   5. Staining techniques

3. BIOCHEMICAL TESTS

4. GROWTH CURVE

5. INDUCTION OF MUTATIONS
   1. Physical mutagenesis
   2. Chemical mutagenesis

6. MUTATIONAL STUDIES BY REPLICA PLATING

7. BACTERIAL CONJUGATION

8. SCREENING FOR DESIRED ORGANISMS

9. ISOLATION AND IDENTIFICATION OF FUNGI

10. PRODUCTION OF SECONDARY METABOLITES

11. ANALYSIS OF METABOLITES
    1. Chromatography
    2. Solvent extraction

12. ANTIBIOTIC ASSAY

13. TITRIMETRIC ESTIMATION OF ORGANIC ACID

14. SPECTROPHOTOMETRIC ESTIMATION OF ENZYME PROTEIN

15. MICROBIAL ANALYSIS OF MILK

16. WATER ANALYSIS

MODULE II:

1. INTRODUCTION TO INDUSTRIAL MICROBIOLOGY

2. BASIC CONCEPTS
   1. Sterilization of fermentation equipment
   2. Fermentation systems
   3. Steps in fermentation
   4. Designing of production media
   5. Development and transfer of seed

3. SCREENING OF DESIRED ORGANISMS

4. TYPES OF FERMENTATIONS
   1. Solid state fermentation
   2. Submerged fermentation

5. DOWN STREAM PROCESSING
   1. CELL SEPERATION TECHNIQUES
   2. EXTRACTION OF DESIRED PRODUCTS
   3. PURIFICATION OF PRODUCTS

6. ANALYSIS OF METABOLITES OR PRODUCTS
   1. Chromatography
   2. Spectrophotometric determination
   3. Titrimetric estimation
   4. Electrophoresis

7. WATER ANALYSIS

8. ENZYME PRODUCTION
   1. Solid sate fermentation
   2. Submerged fermentation

9. ENZYME PURIFICATION TECHNIQUES
   1. Precipitation methods

10. CHROMATOGRAPHY

11. ENZYME ASSAY

12. QUANTITATIVE ESTIMATION OF PROTEINS

13. ENZYME KINETICS
    1. pH
    2. Temperature
    3. Substrate concentration
    4. Activators
    5. Inhibitors

14. ELECTROPHORESIS TECHNIQUES
    1. SDS-PAGE
    2. Native PAGE
    3. Zymography

15. IMMOBILIZATION ENZYMES

MODULE III:

RECOMBINANT DNA TECHNOLOGY

1. FUNDAMENTALS
1. Isolation of Genomic DNA
2. Isolation of Total RNA
3. Quantitative and Qualitative analysis of Nucleic acids
4. Transformation
5. Isolation of Plasmid DNA
6. Agarose Gel Electrophoresis
7. Restriction Digestion
8. PCR Techniques

MODULE IV:

1. INTRODUCTION TO IMMUNOLOGY

2. AGGLUTINATION REACTIONS                        
   1. Blood grouping and Rh typing
   2. Rapid plasma reagin test (VDRL)
   3. Pregnancy test

3. GEL DIFFUSION TECHNIQUES
   1. Ouchterlony Double Diffusion (ODD)
   2. Single Radial Immuno Diffusion (RID)

4. IMMUNO ELECTROPHORESIS
   1. Counter Current Electrophoresis (CCE)
   2. Rocket Immuno Electrophoresis (RIE)

5. ISOLATION OF IgG FROM BLOOD

6. PURIFICATION OF IgG
   1. Salt precipitation
   2. Chromatography
   3. Quantitative estimation of proteins
   4. SDS PAGE

7. SEPERATION OF LYMPHOCYTES

8. ELISA (STRIP TEST)

9. RAISING OF POLYCLONAL ANTIBODIES

10. MONOCLONAL ANTIBODY PRODUCTION

MODULE V:

1. INTRODUCTION TO MEDICAL BIOTECHNOLOGY

2. BASIC CONCEPTS

3. ISOLATION OF PATHOGENS

4. BIOCHEMICAL TESTS (specialized test if any)

5. ANTIBIOTIC SENSITIVITY

6. DIAGNOSTIC TESTS
   1. C - reactive protein (CRP)
   2. Rapid Plasma Reagin (RPR)
   3. VDRL
   4. WIDAL

7. REVIEW OF ANALYSIS

8. REPORT PREPARATION

9. BIOCHEMICAL TESTS
   1. Blood grouping and Rh typing
   2. Hemoglobin test
   3. Pregnancy test

10. URINE ANALYSIS
    1. Detection of urine protein
    2. Detection of urine glucose
    3. Detection of ketone bodies in urine sample

11. WBC Count

12. MIC and MLC

13. INTRODUCTION TO BIOINFORMATICS