PREPARATORY MODULE

Duration - 15 days

ISOLATION OF MICROORGANISMS FROM SOIL SAMPLE BY SERIAL DILUTION PURE CULTURE PREPARATION SCREENING FOR THE ENZYME PRODUCTION ENZYME PURIFICATION Salt precipitation CHROMATOGRAPHY QUANTITATIVE ESTIMATION OFPROTEINS ENZYME KINETICS Temperature pH IMMOBILIZATION OF ENZYME

BASIC MODULE

Duration - 30 Days

ISOLATION OF BACTERIA FROM DIFFERENT SOIL SAMPLE BY SERIAL DILUTION. PURE CULTURE PREPARATION BATCH CULTURE FERMENTATION solid state fermentation submerged fermentation ENZYME PURIFICATION METHODS salt precipitation QUANTITATIVE ESTIMATION OF PROTEINS. ENZYME KINETICS pH Temperature Substrate concentration SDS-PAGE ZYMOGRAPHY (THEORY)

ADVANCE MODULE

Duration - 60 Days

ISOLATION OF BACTERIA FROM DIFFERENT SOIL SAMPLE BY SERIAL DILUTION PURE CULTURE PREPARATION FERMENTATION Solid sate fermentation Submerged fermentation ENZYME PURIFICATION TECHNIQUES Precipitation methods PROTEIN SEPARATION BY CHROMATOGRAPHY ENZYME ASSAY QUANTITATIVE ESTIMATION OF PROTEINS ENZYME KINETICS Ph Temperature Substrate concentration Activators Inhibitors ELECTROPHORESIS TECHNIQUES SDS-PAGE Native PAGE Zymography IMMOBILIZATION ENZYMES